Amondys 45
casimersen · DMD exon 45 (PMO)Dose-related complement activation (Bb, C3a) in 12-week monkey; no immune function effects in juvenile rat
Oxlumo: which risks did reviewers probe?
lumasiran · HAO1 (siRNA)
Immune & cytokine safety
Low-titre ADA in 3/36 monkeys, no impact on exposure or tox; no ADA in rats; not immunostimulatory
“A low incidence, 3 out of 36 treated monkeys in the 36- week repeat-dose toxicity study (GO1-GLP15-036) were confirmed to have a low titre of anti-lumasiran antibodies.”
Biodistribution & persistence
Rat: liver, kidney cortex/medulla highest; not detected in brain/spinal cord; long liver half-life (585 h) in monkey
“Relatively high tissue concentrations (in descending order) were observed in the liver, kidney cortex, kidney medulla, cecum, salivary gland, bone marrow, thyroid and pancreas.”
Modality-specific risk
Rodent-specific hepatocellular changes (vacuolation, necrosis); renal basophilic granules; GalNAc-siRNA class effects (ISR, LFTs, ADA)
“AEs or toxicities associated with GalNAC-conjugated siRNA products include injection site reactions, ALT and AST elevations, mild increases in serum creatinine and decreases in eGFR, positive ADA, and hypersensitivity (including anaphylactic reactions).”
Same question, other oligonucleotides
Amvuttra
vutrisiran · TTR (siRNA)No ADA in rats; non-dose-related ADA in 8/32 monkeys without impact; no cytokine stimulation in vitro or in monkeys
Dawnzera
donidalorsen · Prekallikrein / KLKB1 (ASO)No cytokine/chemokine or C3 effects; minimal transient complement Bb increase at 30 mg/kg/week; ADAs 12.5-62.5% in monkey without impact
Exondys 51
eteplirsen · DMD exon 51 (PMO)No effects on TDAR or immunophenotyping in juvenile rat; lymphoid necrosis in spleen/thymus at high dose in mouse
Givlaari
givosiran · ALAS1 (siRNA)Low ADA incidence: rat negative, one monkey ADA-positive; no dedicated antigenicity studies
Izervay
avacincaptad pegol · C5 (aptamer)Main pharmacology-related risk is increased susceptibility to infections (C5 inhibition); no complement activation by the aptamer in monkeys; panuveitis with Lucentis combination
Kynamro
mipomersen · APOB (ASO)Class proinflammatory effects (MCP-1, IL-1b, CRP); alternative complement activation, C3 depletion in monkeys; no clear ADA
Leqvio
inclisiran · PCSK9 (siRNA)Delayed/suppressed TDAR (KLH IgM/IgG) in 40-week monkey study, reversible; sporadic low-titre ADA without PD impact
Macugen
pegaptanib · VEGF-165 (aptamer)No local/systemic immune response or antibody induction; antibody assay of unclear accuracy
Onpattro
patisiran · TTR (siRNA)Transient complement activation (C3a, Bb) in monkeys, siRNA-independent; ADA to PEG lipid in rats reduced exposure
Qalsody
tofersen · SOD1 (ASO)CSF inflammation at 35 mg in monkey; ADA not assessed in animal studies (58.4% of patients ADA-positive)
Qfitlia
fitusiran · Antithrombin / SERPINC1 (siRNA)No ADA in monkeys (0.1-1 mg/kg/week); injection-site hypersensitivity-type skin reactions in rats and monkeys at all doses
Redemplo
plozasiran · APOC3 (siRNA)Proinflammatory cytokines induced in human whole blood in vitro at high concentrations; none in rat/monkey studies
Rivfloza
nedosiran · LDHA (siRNA)No cytokine or complement activation in 39-week monkey study; nonclinical ADA not assessed
Rytelo
imetelstat · Telomerase (oligo)Alternative complement activation (Bb, C5a); no immunotoxicity; no ADA assessment in animals
Spinraza
nusinersen · SMN2 (ASO)No complement activation or systemic inflammation in IT monkey studies; proinflammatory effects in mice
Tegsedi
inotersen · TTR (ASO)Monkey perivascular inflammation with ADA, CRP, IL-6, TNFa, IgG/IgM rises; complement Bb activation; ADA in some monkeys
Tryngolza
olezarsen · APOC3 (ASO)Complement activation (Bb up, C3 down) in monkeys; MCP-1/CRP/IFN-gamma increases; ADAs in 25-62.5% of monkeys
Viltepso
viltolarsen · DMD exon 53 (PMO)No cytokine or immunophenotype effects; transient ~2-fold C3 rise at high dose in mouse; no anti-drug antibodies
Vyondys 53
golodirsen · DMD exon 53 (PMO)Transient complement activation (Bb, C3a) in 12-week monkey; no immune function effects in juvenile rat
Wainua
eplontersen · TTR (ASO)Transient complement Bb increases; non-neutralising ADAs in chronic monkey study; minimal cytokine changes
Waylivra
volanesorsen · APOC3 (ASO)Complement activation in monkeys (class effect, species-specific); cytokine/chemokine increases (MCP-1, MIP-1b); anti-volanesorsen antibodies in 50-72% of monkeys
Zanvastro
zilganersen · GFAP (ASO)Low-severity proinflammatory findings (liver mononuclear infiltrates, vacuolated macrophages) in 26-week SC mouse study